Lipid Profiling by Electrospray Ionization Tandem Mass Spectrometry and the Identification of Lipid Phosphorylation by Kinases in Potato Stolons

Lipid profiling of FPLC-separated lipoprotein fractions by electrospray ionization tandem mass spectrometry.

Glycerophospholipid and sphingolipid species and their bioactive metabolites are important regulators of lipoprotein and cell function. The aim of the study was to develop a method for lipid species profiling of separated lipoprotein classes. Human serum lipoproteins VLDL, LDL, and HDL of 21 healthy fasting blood donors were separated by fast performance liquid chromatography (FPLC) from 50 mic...

Profiling histidine-containing dipeptides in rat tissues by liquid chromatography/electrospray ionization tandem mass spectrometry.

The histidine-containing dipeptides carnosine (CAR) and structurally related anserine (ANS) and homocarnosine (HCAR), widely distributed in vertebrate organisms, have recently been proposed as endogenous quenchers for highly cytotoxic alpha,beta-unsaturated aldehydes generated by peroxidation. A sensitive, selective, specific and rapid liquid chromatographic/electrospray ionization tandem mass ...

chromatography–electrospray ionization tandem mass spectrometry

Amino Acid Profiling by Reactive Desorption Electrospray Ionization Tandem Mass Spectrometry (DESI-MS/MS)

Desorption electrospray ionization mass spectrometry (DESI MS) has gained significant recognition over the past few years because of its simplicity and rapid throughput capabilities, allowing for the direct analysis of samples with a wide variety of sizes, shapes, and chemistries. Addition of various reagents into the DESI spray solvent enables atmospheric pressure ion molecule reactions betwee...

Quantitative profiling method for oxylipin metabolome by liquid chromatography electrospray ionization tandem mass spectrometry.

Cyclooxygenase, lipoxygenase, and epoxygenase derived oxylipins, especially eicosanoids, play important roles in many physiological processes. Assessment of oxidized fatty acid levels is important for understanding their homeostatic and pathophysiological roles. Most reported methods examine these pathways in isolation. The work described here employed a solid phase extraction-liquid chromatogr...